Journal:

Article Title: Identification of a New Repetitive Element in Staphylococcus aureus

doi:

Figure Lengend Snippet: Database sequence entries

Article Snippet: Length (bp) of STAR element No. of signature sequences uvrA-hprK star01 601055 1,127 {"type":"entrez-nucleotide","attrs":{"text":"AF195957","term_id":"7330751"}} AF195957 434 5 star02 8325-4 876 {"type":"entrez-nucleotide","attrs":{"text":"AF195958","term_id":"7330754"}} AF195958 279 3 star03 ATCC 10832 825 {"type":"entrez-nucleotide","attrs":{"text":"AF195959","term_id":"7330757"}} AF195959 226 2 star04 DSM 20232 720 {"type":"entrez-nucleotide","attrs":{"text":"AF195960","term_id":"7330760"}} AF195960 109 1 star05 ATCC 49834 940 {"type":"entrez-nucleotide","attrs":{"text":"AF195961","term_id":"7330763"}} AF195961 329 3 star06 ATCC 12601 573 {"type":"entrez-nucleotide","attrs":{"text":"AF195962","term_id":"7330766"}} AF195962 0 0 icaC-geh star07 ATCC 10832 521 {"type":"entrez-nucleotide","attrs":{"text":"AF195963","term_id":"7330769"}} AF195963 168 2 star08 601055 494 {"type":"entrez-nucleotide","attrs":{"text":"AF195964","term_id":"7330772"}} AF195964 157 3 star09 ATCC 49834 380 {"type":"entrez-nucleotide","attrs":{"text":"AF195965","term_id":"7330775"}} AF195965 43 1 star10 ATCC 12601 464 {"type":"entrez-nucleotide","attrs":{"text":"AF195966","term_id":"7330778"}} AF195966 108 1 star11 ATCC 35556 577 {"type":"entrez-nucleotide","attrs":{"text":"AF195967","term_id":"7330781"}} AF195967 220 3 Open in a separate window Database sequence entries fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window FIG. 2 caption a7 Comparison of sequenced STAR elements.

Techniques: Sequencing

Journal: bioRxiv

Article Title: JNK regulates GABAAR expression at the cell surface via the receptor clustering protein GIT1 (ArfGAP1)

doi: 10.1101/2025.01.22.634236

Figure Lengend Snippet: A. In vitro phosphorylation of brain homogenate by recombinant active JNK1 revealed phosphorylation of GIT1 on S371 and S692. Mass spectrometry-identified sequences are shown for GIT1 (accession NP_001078923.1). Β. Representative autoradiograph and Coomassie Brilliant Blue (CBB) stained gel image are shown for recombinant GST-GIT1- wild-type (WT), -S371A, -S692A or S371A/S692A (SAA) variants after phosphorylation by GST-JNK1. JNK1 phosphorylated GIT1-WT and GIT1–S692A but not GIT1-S371A, indicating that S371 is the preferred JNK1 phosphorylation site. Phosphorylation of GIT1 by JNK1 was prevented by JNK inhibitor 10 µM SP600125. C. Quantitative data from 4 repeats of experiment shown in B . D. Domain map of human GIT1 (UniProt identifier: Q9Y2X7-3) shows predicted JNK1 phosphorylation sites (yellow circles). Abbreviations: Arf GTPase activating protein domain (GAP), 3x Ankyrin repeats (ANK), Spa-Homology domain (SHD), Synapse Localization Domain (SLD) and Paxillin Binding Domain (PBD). E. Quantitative LC-MS/MS data of GIT1-S371 phosphorylation in wild-type (WT) and Jnk1-/- whole brains. F. Quantitative LC-MS/MS data of GIT1-S371 phosphorylation in brains from DJNKI-1- infused adult mice. G. Active Arf1 was detected from pull downs using GST-GAT-GGA3 immobilized on glutathione beads. Cells expressed GIT1-variants together with Arf1-WT or constitutively active Arf1-Q71L. H. Quantitative data from 3 repeats of experiment described in G. Phosphorylation-site mutants of GIT1 did not alter Arf1 activity. Error bars represent standard error of the mean (SEM) and P-values were determined using Student’s t test.

Article Snippet: For in vitro phosphorylation assay, pEBG-JNK1α1 and pEGFP-MEKK1Δ(1174-1493) have been previously described ( ). pmRuby2-Lifeact7 was from Michael Davidson’s lab via Addgene, pEGFP-Dynamin 2-WT and pEGFP-Dynamin 2-K44A was from Pietro De Camilli. pcDNA3-HA-Arf1 (Addgene plasmid # 10830) and pcDNA3-HA-Arf1-ActQ71L (Addgene plasmid # 10832) were generated by Thomas Roberts. pGEX-5x-1-VHS GAT-GGA3 was a gift from Juan S. Bonifacino. pEGFP-PXN-WT was described previously ( ).

Techniques: In Vitro, Recombinant, Mass Spectrometry, Autoradiography, Staining, Binding Assay, Liquid Chromatography with Mass Spectroscopy, Activity Assay